Optimization of Reflux Extraction Technology of Hyperin in Pine Needle of Cedrus Deodra by Response Surface Methodology

OBJECTIVE To optimize the reflux extraction technology of hyperin in pine needle of Cedrus deodra. METHODS The content of hyperoside in pine needle of Cedrus deodara was determined by HPLC. On the basis of single factor test, the hyperoside content was used as indexes, three factors (solid-to-liquid ratio, ethanol concentration and extraction time) were studied by Box-Behnken design, the reflux extraction technology of hyperin in pine needle of Cedrus deodra was optimized by response surface methodology. RESULTS The optimal parameters of reflux extraction technology were as follows:ethanol concentration was 60%, solid-to-liquid ratio was 1:16(g·mL^-1), extraction time was 100 min for once. Under the above conditions, the average content of hyperin was 0.119 6 mg·g^-1, which was similar to the predicted value 0.119 4 mg·g^-1. CONCLUSION The preferred extraction technology of hyperin in pine needle of Cedrus deodra is reasonable and feasible.