Determination of Eight Glycosides in Compound Ginseng Tablets by QAMS

OBJECTIVE To establish a quantitative of multi-components by single maker for the content determination of eight glycosides in Compound Ginseng tablets.METHODS The analysis of methanol extract of this drug was performed on a 25℃ Agilent XDB -C₁₈ column(4.6 mm×250 mm, 5 μm), with the mobile phase of water(A)-acetonitrile(B) flowing at 1.0 mL·min^-1 in a gradient elution manner, and the detection wavelength was set at 203 nm. Ginsenoside Rb1 was used as the internal reference substance, RCFs of ginsenoside Rg1, ginsenoside Re, icarrin, ginsenoside Rc, ginsenoside Rb2, ginsenoside Rb3, ginsenoside Rd was calculated. The contents of seven consitituents were determined by both external standard and QAMS. The validity of the QAMS method was evaluated by comparison of their quantitative results of both methods.RESULTS Eight consitituents showed good linear relationships within their own ranges (r ≥ 0.999 0), whose average recoveries were 95.6%-104.4% with the RSDs of 1.22%-2.73%. The results obtained by QAMS method approximated those obtained by external standard method.CONCLUSION The method of QAMS turns out to be simple, sensitive, accurate, specific and reproducible in the quality control of Compound Ginseng tablets.