Study on Content Determination of Main Flavonoid Glycosides in Different Medical Parts of Caragana Altaica Kom. Pojark

OBJECTIVE To determine the content of rutin and kaempferol-3-O-rutinoside in the roots, stems, leaves, flowers and fruits of Caragana altaica kom. pojark from Xinjiang Altai Region.METHODS HPLC conditions:Inertsil ODS-3 column(250 mm×4.6 mm, 5 μm), detection wavelength at 254 nm, flow rate at 1.0 mL·min^-1, injection volume was 5 μL and column temperature was 25℃. A gradient elution of 0.2% phosphoric acid (A) and acetonitrile (B) solution were performed with following procedure:0-5 min, 10%→14% B; 5-10 min, 14%→17% B; 10-16 min, 17%→20% B; 16-21 min, 20%→22% B; 21-30 min, 22%→25% B; 30-40 min, 25% B.RESULTS The average content of rutin in 10 batches of fruits, leaves and flowers was 6.83, 6.16, 3.58 mg·g^-1 and the average content of kaempferol-3-O-rutinoside was 1.48, 2.44, 1.01 mg·g^-1, respectively. However, neither rutin nor kaempferol-3-O-rutinoside were detected in the roots and stems of Caragana altaica kom. pojark.CONCLUSION The content determination method established in this study is simple and showes good stability and repeatability. Furthermore, this study provides experimental basis for the study of flavonoid glycosides in Caragana altaica kom. pojark. and provides essential experimental data for the establishment of quality standard of that plant.