Optimization of Ultrasound-assisted Extraction Technology of Canthin-6-one from Bark of Ailanthus Altissima by Response Surface Methodology

OBJECTIVE To optimize ultrasonic-assisted extraction technology of canthin-6-one from bark of Ailanthus altissima.METHODS With yield of canthin-6-one as index, extraction technology parameters were optimized by central composite design-response surface methodology on the basis of the single factor investigation of conditions for extracting canthin-6-one by ethanol.RESULTS Optimum extraction conditions were as follows:ethanol concentration 68%, liquid-solid ratio 32 mL·g^-1, ultrasound temperature 54℃, extraction time 38 min. Under these conditions, yield of canthin-6-one was 26.19 μg·g^-1, which was close to the predicted value(26.31 μg·g^-1).CONCLUSION This optimized technology is reasonable and reliable to provide a basis for development and application of Ailanthus altissima.