Research Progress of Extraction, Isolation, Purification and Bioactivities of Polysaccharides from Cyclocarya paliurus

OBJECTIVE To analysis the extraction, isolation, purification and biological activities of Cyclocarya paliurus polysaccharide (CPP), given the reference for the further research and development of it. METHODS Literatures on extraction, isolation, purification and biological activities of CPP in recent 10 years were summarized and analyzed. RESULTS Currently, the methods for CPP extraction included ultrasonic extraction, microwave assisted extraction, reflux extraction, hot water extraction, ultrasonic enzymatic and ultra pressure extraction; the methods of content determination include phenol sulfuric acid, anthrone sulfuric acid, UV spectrophotometry, HPLC and DNS colorimetric; the methods of separation and purification include ion exchange resin, gel filtration column chromatography and affinity chromatography. CONCLUSION Each methods have it's advantages and disadvantages, to be reasonable selection according to the research purpose andconditions. The biological activities of CPP has achieved certain results, but only limited to the crude extract. For the development of more drug formulations and related products to be further studied.