Simultaneous Determination of Seven Constituents in Citrus changshanhuyou Y. B. Chang by HPLC

OBJECTIVE To establish a method for the simultaneous determination of the contents of narirutin, naringin, hesperidin, neohesperidin, luteolin, nobiletin and hesperetin of Citrus changshan-huyou Y.B.Chang by HPLC. METHODS The HPLC method was adopted with Agilent Extend C₁₈ column (250 mm×4.6 mm, 5 μm). The mobile phase was acetonitrile and 0.1% methanoic acid with gradient elution. The flow rate was 1 mL·min^-1. The detection wavelength was 330 nm and column temperature was 30℃. RESULTS The linear relation of narirutin, naringin, hesperidin, neohesperidin, luteolin, nobiletin and hesperetin was excellent within the range of 23.30-1 164.80 ng, 170.84-8 541.91 ng, 17.22-861.20 ng, 156.17-7 808.64 ng, 3.13-156.48 ng, 0.90-45.23 ng, 0.85-42.27 ng (r ≥ 0.999 5, n=6), and the mean recovery was 98.8%, 101.3%, 98.3%, 96.8%, 101.8%, 101.7%, 108.9%. CONCLUSION It is the first time to establish the HPLC method of the simultaneous determination for the seven contents of Citrus changshan-huyou Y.B.Chang. The method is simple, reliable and accurate, which provides the reference for the quality control for Citrus changshan-huyou Y.B.Chang.