Simultaneous Determination of Tenacissoside A, Tenacissoside H and Tenacissoside I in Xiaoaiping Injection by UPLC-MS/MS

OBJECTIVE to establish a method for the simultaneous determination of Tenacissoside A, Tenacissoside H and Tenacissoside I in Xiaoaiping injection by UPLC-MS/MS. METHODS The separation was performed on a Phenomenex Kinetex XB-C₁₈ column(2.1 mm×50 mm, 2.6 μm) using the mobile phase of acetonitrile-0.1% formic acid solution at a flow rate of 0.2 mL·min^-1 in gradient elution mode. The detection was performed on a triple quadrupole tandem mass spectrometer by multiple reaction monitoring via electrospray ionization source in positive mode. RESULTS The linearity range of Tenacissoside A, Tenacissoside H and Tenacissoside I were 0.05-10 ng·mL^-1, 0.025-10 ng·mL^-1, 0.025-10 ng·mL^-1, respectively, with all correlation coefficient (r) >0.998. The limit detection was 0.012 5-0.025 ng·mL^-1 and the recoveries were 97.9%, 95.7%, 96.1%, respectively, with RSD <3.4%. CONCLUSION The proposed UPLC-MS/MS method is simple, rapidness, accurate, and sensitivity, can be used for the quantitative determination of Tenacissoside A, Tenacissoside H and Tenacissoside I in Xiaoaiping injection.