Fingerprint Analysis and Determination of Flavonoid Extract from Mosla Scabra

OBJECTIVE To establish the method of fingerprint analysis and determination of flavonoids from Mosla scabra(MSF) by HPLC. METHODS HPLC separation was carried out on Hypersil BDS C₁₈ (250 mm×4.6 mm, 5 μm) column with a gradient elution consisted of methanol and 0.1% phosphoric acid aqueous solution. The flow rate was kept at 1.0 mL·min^-1. Column temperature was kept constantly at 30℃. The UV absorbance was monitored at 270 nm. RESULTS Nine batches of MSF were collected to generate representative chromatographic fingerprints. Thirteen common peaks were defined in characteristic fingerprints and five selected marker compounds in MSF were well identified by comparing the retention times of the reference standards. The similarity values of 9 samples were all higher than 0.96. Reasonable linear regressions of cynaroside, luteolin, apigenin, kaempferol and 7-methoxy wogonite were obtained in the fingerprints at ranges of 19.42-194.2 μg·mL^-1, 0.520 0-5.200 μg·mL^-1, 0.214 0-2.140 μg·mL^-1, 0.134 4-1.344 μg·mL^-1 and 0.013 60-0.136 0 μg·mL^-1, respectively. Average recovery rates of five compounds were 100.31%, 98.94%, 99.15%, 100.95% and 98.57%, respectively, with the RSD of less than 3% (n=5). CONCLUSION The method is reproducible and precise and can be used for convenient quality assessment of Mosla scabra.