Determination of Dissolution and Content of Two-dimensional Rutin Tables by HPLC

OBJECTIVE To determine dissolution and content of rutin in two-dimensional rutin tablets by HPLC. METHODS The dissolution medium of determination of dissolution was water (containing 0.5% SDS). Apparatus 2 was used with 75 r·min^-1. The dissiution medium was taken and analyzed by HPLC at 45 min. Determination of content was measured using a Thermo C₁₈ column. The mobile phase was treated with phosphate buffer (0.l mol·L^-1 sodium dihydrogen phosphate solution and adjusted to pH 4.4 with phosphoric acid)-acetonitrile (80:20). The detection wavelength was 254 nm. RESULTS Dissolution determination:it showed that stardard curve was linear in the range of 2.26-22.56 μg·ml^-1(r=1.000 0). The average recovery was 98.2% (RSD=0.4%) for rutin. The disslution of two-dimensional rutin tablets from three batches were >75%. Determination of content:the method for determination of rutin had a good linearity, and the recovery was 100.6%(RSD=0.4%, n=9). CONCLUSION The method is simple, sensitive and reproducible, and it can be used for the determination of dissolution and content of two-dimensional rutin tablets.