Determination of Ginkgolic Acids in the Ginkgo Leaf

OBJECTIVE To develop an HPLC method for the determination of ginkgolic acids content in the Ginkgo leaf. METHODS The sample was extracted by ultrasonic extraction with methanol as the solvent, the separation was performed on C₁₈ column(4.6 mm×150 mm, 5 μm), with 0.1% trifluoroacetic acid solution of acetonitrile(A)-0.1% trifluoroacetic acid aqueous solution(B) as mobile phase, and eluted with a gradient program(0-30 min, 75%A→90%A; 30-35 min, 90%A; 35-36 min, 90%→75%A; 36-45 min, 75%A), flow rate 1 mL·min^-1, and UV detection wavelength at 310 nm. RESULTS It was convenient to prepare the sample of Ginkgolic acids by ultrasonic extraction with methanol as the solvent. The linear range for the content of ginkgoneolic acid was 0.917-45.85 μg·mL^-1(r²=0.999), the average recovery was 97.34%, and RSD was 0.57%. CONCLUSION The method is sensitive, accurate and speedy, therefore suitable for the determination of Ginkgolic acids content in the Ginkgo leaf.