HPLC Fingerprints Analysis of Processed Aconitum Sinomontanum Nakai and Determination of the Contents of Two Alkaloids

OBJECTIVE To establish an HPLC fingerprint of processed Aconitum sinomontanum Nakai from different areas, and determine the contents of two alkaloids to evaluate the quality of the preparation. METHODS HPLC gradient elution method were developed to establish fingerprints for processed Aconitum sinomontanum Nakai, and the fingerprint were analyzed and compared by Chinese Materia Medica (CMM) Fingerprint Similarity Evaluation System (2012 edition), principle component analysis and cluster analysis. RESULTS The common mode for processed Aconitum sinomontanum Nakai fingerprint was established, the similarity was greater than 0.90 in 10 batches of processed Aconitum sinomontanum Nakai medicinal herbs, 18 common fingerprint peaks were identified, and the content of lappacontine and ranaconitine were determined. The different areas of samples could be classified into four groups by using cluster analysis, which reflected the quality characteristics of 10 batches of processed Aconitum sinomontanum Nakai from different areas. We screened four main components whose cumulative contribution rate were 88.8% by principle component analysis, and get seven chemical composition which determined the quality of processed Aconitum sinomontanum Nakai. CONCLUSION This method has good reproducibility, and strong characteristic, which can be used for the quality evaluation of processed Aconitum Sinomontanum Nakai.