Improvement of Content Determination of Baibei Yifei Capsule

OBJECTIVE To augment evaluation index, and establish a scientific and reasonable method for the content determination of Baibei Yifei capsule. METHODS Samples were purified by C₁₈ column, optimized chromatographic conditions:Vp-ODS column (150 mm×4.6 mm, 5 μm); column temperature:20℃; fluctuan flow rate; the mobile phase acetonitrile (A)-water (B), gradient elution. The injection volume was 10 μL. The detection wavelength was 203 nm. RESULTS The contents of notoginsenoside R₁, ginsenoside Rg₁, ginsenoside Rb₁ were linear in 40.24-241.44(r=0.999 6), 91.50-549.00 (r=0.999 9), 35.72-241.32(r=0.999 8)mg·mL^-1, respectively. The recovery rate was 94.15%-99.81%, RSD was 1.12%-1.94%. CONCLUSION The method is accurate and reproducible, which can provide the basis for the revision of the standard.