Study of Quality Control on Compound Angelica Fuyan Micro Enema

OBJECTIVE To establish a method of quality control for Compound Angelica Fuyan Micro Enema. METHODS According to the composition of prescription, the qualitative identification of Angelicac Sinensis Radix, Paeoniae Radix Rubra and Corydalis Rhizoma in Compound Angelica Fuyan Micro Enema was performed by TLC. The content of ferulic acid and paeoniflorin in Compound Angelica Fuyan Micro Enema was determined by HPLC:using Extend-C₁₈ (4.6 mm×250 mm, 5 μm) column; acetonitrile-0.1% phosphoric acid (15:85) was as the flow equality. Column temperature was 25℃; flow velocity 1 mL·min^-1; the detection wavelength was 320, 234 nm. RESULTS Ferulic acid and paeoniflorin had good linear relationship within 0.002 4-0.011 9 mg·mL^-1(r=0.999 4), 0.036 4-0.081 9 mg·mL^-1(r=0.999 2). The average recovery rate was 100.3%, 98.9% and the RSD was 1.08%, 0.68% respectively. CONCLUSION The established method is simple, accurate and stable well, and can be used for the quality control of the preparation.