Determination of Calcium Pantothenate and Hydrochlorothiazide in Compound Kendir Leaves Tablet Ⅰ by Dual Wavelengths of HPLC

OBJECTIVE To establish a dual wavelengths of HPLC method for the determination of calcium pantothenate and hydrochlorothiazide in Compound Kendir Leaves tablet Ⅰ. METHODS The Kromasil C₁₈ (250 mm×4.6 mm, 5 μm) was used with the mobile phase A0.1% octane sulfonic acid sodium (adjust pH to 3.0 with phosphoric acid)-acetonitrile (964)-methanol as mobile phase B with gradient elution. The column temperature was set as 35℃ and the detection wavelength was set at 200 nm for calcium pantothenate and 275 nm for hydrochlorothiazide. The flow rate was 1.0 mL·min^-1 and the injection volume was 10 μL. RESULTS The linear range was good within 0.025-0.5 μg (r=0.999 6) for calcium pantothenate, 0.16-3.2 μg(r=1.000 0) for hydrochlorothiazide, respectively. The average recoveries(n=9) were 101.2% (RSD=0.47%) and 101.6% (RSD=0.49%), respectively. CONCLUSION The method is accurate and reliable for the quality control of this compound preparation.