Response Surface Methodology for Optimization of Extraction Process of Naringin and Neohesperidin from Huyou fruitlet

OBJECTIVE To optimize the extraction process of naringin and neohesperidin from huyou fruitlet. METHODS Based on the single factor experiment, Box-Benhnken three factors (ethanol concentration, solid-liquid ratio and extraction time) experiment by response surface design was carried out. RESULTS The optimum conditions was as follows:solid to liquid ratio 1:10, 60% ethanol, extraction time 1.8 h and extraction two times. Under these conditions, the total yield of naringin and neohesperidin from huyou fruitlet was 23.44%. CONCLUSION The method is simple, reasonable, feasible and provide a experiment basis for its industrial production.