Development of PCR Method Using TaqMan Probe for the Detection of Human Bocavirus and Investigation of Pollution of Blood Products and Raw Plasma

OBJECTIVE To develop a quick and convenient method for detection of human bocavirus (HBoV), and investigate the pollution situation of blood products and raw plasma in south of China. METHODS Real time fluorescence quantitative PCR using TaqMan probe was established. Blood products collected from different manufacturer including original single plasma and raw plasma samples, serum albumin, intravenous injection of immune globulin protein (IVIG), fibrin raw and human clotting factor Ⅷ and human fibrinogen fibrin glue to human fibrin were checked. RESULTS The detection rate of HBoV-DNA in raw plasma samples and human clotting factor Ⅷ were 5.2% and 5.0% respectively. But no HBoV-DNA was detected in other blood products. CONCLUSION PCR method developed in this thesis is qualified to test HBoV-DNA in blood products and raw plasma, which is of great significance to the standardized production and medication safety.