Study on UPLC Fingerprint of Zhibai Dihuang Pills

OBJECTIVE To develop a method for fingerprint study of Zhibai Dihuang pills by UPLC. METHODS The chromatographic condition was as follows:Waters Acquity BEH C₁₈ column(2.1 mm×100 mm, 1.7 μm), using acetonitrile-0.1% H₃PO₄ as mobile phase, with a flow rate of 0.4 mL·min^-1, the detective wavelength was set at 236 nm and the column temperature was 40℃. Paeonol was used as the reference substance. RESULTS The analysis time was 12 min. There were 12 common peaks in the fingerprints, 6 peaks were identified and the similarity of the fingerprint was compared. CONCLUSION The method is simple, feasible, and can be used for qulity control of Zhibai Dihuang pills.