Study on the Stability and Antioxidant Activity of 3,5-O-Dicaffeoylquinic Acid

OBJECTIVE To investigate the factors influencing the stability of 3,5-O-dicaffeoylquinic acid and investigate the antioxidant activity of 3,5-O-dicaffeoylquinic acid in vitro. METHODS The stability of 3,5-O-dicaffeoylquinic acid in different solvents, temperatures, pH values, and illuminations were measured by HPLC. And its antioxidant activity (DPPH free radical scavenging capacity) was measured by UV. RESULTS The stability of 3,5-O-dicaffeoylquinic acid was influenced by the solvent, pH value, temperature and illumination. 3,5-O-dicaffeoylquinic acid showed strong DPPH free radical scavenging capacity3,5-O-dicaffeoylquinic acid's IC₅₀ value was (372.56±1.04)μg·mL^-1, Vc's was (294.54±1.03)μg·mL^-1. CONCLUSION It is recommended to be stored in acidic aqueous solution under low temperature and light protection condition during the separation, purification and analytical process. 3,5-O-dicaffeoylquinic acid will have broad application prospects in pharmaceutical, foodstuff, cosmetics and fine chemicals industries as an antioxidant.