Simultaneous Determination of Four Kinds of Flavonoids in Clematis Intricata Bunge of Mongolian Medicinal Herb by HPLC

OBJECTIVE To establish a method to control the quality of Clematis intricata Bunge through the content determination of the four kinds of flavonoids in Clematis intricata Bunge of Mongolian medicinal herb. METHODS The HPLC analysis was carried out on YMC C₁₈ column(4.6 mm×250 mm, 5 μm) with a methanol-0.04% phosphoric acid solution as the mobile phase for gradient elution at a flow rate of 1.0 mL·min^-1. The detection wavelength was 350 nm, and the column temperature was 25℃. RESULTS Luteoloside, hyperoside, rutin and quercetin exhibited good linear relationships within the ranges of 0.021 37-0.427 4, 0.010 85-0.217 0, 0.021 41-0.428 2, 0.017 46-0.349 2 μg, respectively. The RSD of precision, repeatability and stability tests were <2%, the average recoveries were ranged in 97.4%-102.3%. CONCLUSION The method is simple, accurate and reliable with food repeatability, which can be used for the quality evaluation and control of Clematis intricata Bunge of Mongolian crued drugs.