Research on the Feasibility of Using Chorioallantoic Membrane Vascular Assay to Evaluate Eye Irritation of Cosmetics

OBJECTIVE Study on the feasibility of using Chorioallantoic Membrane Vascular Assay to evaluate eye irritation of cosmetics, and evaluating 14 kinds of cosmetics by improved method. METHODS Establish a standard method on eye irritation using CAMVA of cosmetics by controlling exposure time and sample concentration, and improving the evaluation system. RESULTS Skin care products are non-irritating. There was a positive correlation between exposure time, sample concentration and the stimulation intensity of hair dyes and after using rinse-off cosmetics. At 3% concentration, the best exposure time is 20min for after using rinse-off cosmetics, the classification consistency of CAMVA and Draize test was 90% (9/10), and 5min for hair dyes, the classification consistency of CAMVA and Draize test was 100%(2/2). Irritation can be graded by using the improved evaluation system, the classification consistency of CAMVA and Draize test of after using rinse-off cosmetics was 80% (8/10), and the classification consistency of CAMVA and Draize test of hair dyes was 50%(1/2). CONCLUSION CAMVA method can be applied to predict eye irritation of cosmetics, but the experimental conditions and evaluation system need to be further refined.