Quantitative Analysis of Zhuang Medicine Ilex Pubescens Formula Granules by QAMS

OBJECTIVE To establish a method for simultaneous detemination of six phenolic acids in Ilex pubescens formula granules by quantitative analysis of multi-components by single marker (QAMS). METHODS Chlorogenic acid was used as the internal reference substance. The relative correction factors (RCF) of chlorogenic acid, cryptochlorogenic acid, caffeic acid, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C were established respectively, and their contents in samples were calculated by RCF. At the same time, the external standard method(ESM) was used to detemine the above six phenolic acids. Compared with the content results determined by the ESM and QAMS, the accuracy and feasibility of QAMS method were verified. RESULTS The reproducibility of RCF was acceptable. There was no significant difference between the quantitative results of the above two methods in eight batches of Ilex pubescens formula granules. CONCLUSION The method can be used as quality evaluation method of multi-component in Ilex pubescens formula granules.