Influence of Rutin on Myocardial Ischemia-Reperfusion Injury

OBJECTIVE To explore the effect and mechanism of rutin on myocardial ischemia reperfusion injury in rats. METHODS Forty SD rats were randomly divided into Sham group(Sham), myocardial ischemia reperfusion injury group(IRI) and Rutin groups(Rutin). IRI and Rutin groups were induced IRI by clamping the left anterior descending coronary artery for 0.5 h with noninvasive endoclips. Sham group with sham operation. Rutin groups were pretreated with Rutin(20, 40 mg·kg^-1) at 0.5 h before ischemia by intraperitoneal injection. Rats in IRI group and Sham group were administrated with same dosage saline at the same time. After reperfusion for 4 h, the cardiac tissue and serum were collected. The cardiac pathology was exanimed by HE; The expression of CK-MB, LDH, MCP-1, IL-6, TNF-α in serm were evaluated by ELISA; The expression of PI3K, AKT, p-AKT and m-TOR in heart were evaluated by Western blotting; The expression of MDA and the activity of CAT, GP_X and SOD in heart were examined by TBA and xanthine oxidase method. The expression of IL-6, MCP-1 and TNF-α in serum and cardial tissue were determined by ELISA and RT-PCR. RESULTS Compared with the Sham group, the cardiac morphological change and the expression of CK-MB, LDH, MCP-1, IL-6, and TNF-α in serum, PI3K, p-AKT, mTOR, MDA, MCP-1, IL-6 and TNF-α in heart in IRI group were markedly increased with decreasing the activity of CAT, GP_X and SOD in IRI group. Compared with the IRI group, the cardiac morphological change and the expression of CK-MB, LDH, MCP-1, IL-6, and TNF-α in serum, PI3K, p-AKT, mTOR, MDA, MCP-1, IL-6 and TNF-α in heart were decreased with increasing the activity of CAT, GP_X and SOD in Rutin group. There was no difference on the expression of AKT among three groups. CONCLUSION Rutin can protect rats against myocardial ischemia-reperfusion injury. The mechanism of which is partly by suppressing inflammation and Oxidative stress though promoting the activation of PI3K/AKT/mTOR signal pathway.