Effects of 7-Difluoromethoxy-5,4'-dimethoxygenistein on Adipocyte Cells of Gestational Diabetes Mellitus In-taking Glucose via Up-regulation miR-26b

OBJECTIVE To investigate the effects of 7-difluoromethoxy-5,4'-dimethoxygenistein(DFMG) on adipocyte cells of gestational diabetes mellitus(GDM) in-taking glucose. METHODS Adipocyte cells were extracted from mesenteric adipose tissue of GDM patients. The toxicity of different concentrations of DFMG for adipocyte cells were determined by MTT assay. The rate of adipocyte cells in-taking³H-2-decoxy-glucose was evaluated by liquid scintillation detector. The expression of miR-26b and its downstream target gene PTEN mRNA were examined by RT-PCR assay. The proteins expression related to IRS-PI3K/AKT-mGLUT4 signaling pathways were assessed using Western blotting method. RESULTS There was no toxide side effect of DFMG on akipocyte cells of GDM in vitro. Liquid scintillation detector showed DFMG could prompt the rate of adipocyte cells in-taking³H-2-decoxy-glucosecompared with GDM group(P<0.05). RT-PCR method indicated DFMG could up-regulate the expression of miR-26b and down-regulate its target gene PTEN mRNA compared with GDM group(P<0.05). Western blotting assay showed DFMG could reduce the expression of PTEN protein compared with GDM group(P<0.05), enhance the expressions of p-PI3K, p-AKT and mGLUT4 proteins(P<0.05). CONCLUSION DFMG can promote the rate of adipocyte cells in-taking³H-2-decoxy-glucose via up-regulating miR-26b expression, adjusting the IRS-PI3K/AKT-GLUT4 signaling pathways.