Determination of Glycohyodeoxycholic Acid and Glycochenodeoxycholic Acid in Danyining Tablet by HPLC-ELSD

OBJECTIVE To establish a method for the simultaneous determination of glycohyodeoxycholic acid and glycochenodeoxycholic acid in Danyining tablet by HPLC-ELSD.METHODS The analysis was performed on Welch Ultimate Plus C₁₈(250 mm×4.6 mm,5 μm),the mobile phase consisted of acetonitrile and 0.1% formic acid (40:60).The flow rate was 1.0 mL·min^-1.ELSD6000 was used as the detector,the drift tube temperature was 105℃ and the nitrogen flow rate was 2.5 L·min^-1.RESULTS Glycohyodeoxycholic acid and glycochenodeoxycholic acid had good linear relationships in the ranges of 0.206-3.090 μg (r=0.999 5) and 0.180-2.693 μg (r=0.999 4),respectively.The average recoveries were 99.54%(RSD=0.99%) and 100.58%(RSD=1.70%),respectively.CONCLUSION The method is simple,accurate with good repeatability,which can be used for the quality control of Danyining tablet.