Studies on Fingerprint of Oil of Ganoderma Lucidum Spore and Identification for It's Confusion

OBJECTIVE To establish a specific HPLC fingerprint of the oil of Ganoderma lucidum spore and apply for identifying for its confusion.METHODS The HPLC chromatography was performed on an ACCHROM Unitary-C₁₈ column (250 mm×4.6 mm,5 μm) with the mobile phase of acetonitrile-isopropanol (51︰49).The flow rate was 1.0 mL·min^-1 at 30℃ with the detection of ELSD.RESULTS The chromatographic fingerprint of aliphatic esters of oil of Ganoderma lucidum spore was established.The fingerprint contained 8 recognizable peaks of trilinolein,1,2-linolein-3-olein,1,2-linolein-3-palmitin,1,2-olein-3-linolein,1-palmitin-2-olein-3-linolein,triolein,1,2-olein-3-palmitin and 1,2-olein-3-stearin.The fingerprint of oil of Ganoderma lucidum spore was different from its confution obviously.CONCLUSION The established fingerprint can distinguish oil of Ganoderma lucidum spore from its confusion.