Variance Analysis on the Chemical Constitutes of Different Extracts from Aconitum Soongaricum Stapf.

OBJECTIVE To establish an HPLC method for simultaneously analyzing the alkaloids in Aconitum soongaricum Stapf,and study the chemical differences between ethyl acetate extract and ethanol extract.METHODS The separation was performed on an Agilent TC-C₁₈(2) column (250 mm×4.6 mm,5 μm),and the mobile phase consisted of 0.3% phosphoric acid water (A)-acetonitrile (B) in gradient mode (0-12 min,10%→16% B;12-30 min,16%→35% B;30-40 min,35%→45% B;40-45 min,45% B) at the flow rate of 1.0 mL·min^-1.The detection wavelength was 235 nm,while the column temperature was 35℃ and the injection volume was 20 μL.RESULTS All the alkaloids could be separated completely within 45 min,and the regression equation showed good liner relationship between the peak area and the content of each compound.The ethyl acetate extract contained four alkaloids including hypaconitine,lappaconitine,benzoylaconine and aconitine,while ethanol extract contained five alkaloids including songorine,benzoylaconine,aconitine,bulleyaconitine A and 3-deoxy-aconitine,and the contents of above alkaloids were 1-130 mg·g^-1.Results showed that there were significant differences between the two parts.CONCLUSION This method is simple,accurate,and reproducible,and is suitable for the quality control of Aconitum soongaricum,and can be used for quantitative analysis of other Aconitum crude medicines.