Quantification of Xylooligosaccharides in Chitooligosaccharide Xylooligosaccharide Capsules by HPLC-ELSD

OBJECTIVE To establish an HPLC-ELSD method for the determination of xylooligosaccharide in chitooligosaccharide xylooligosaccharide capsules by amide hydrophilic interaction chromatography.METHODS The chromatographic separation was achieved on a Waters XBridge Amide column (4.6 mm×250 mm, 3.5 µm) by a gradient elution program with acetonitrile-0.2% triethylamine as the mobile phase. The flow rate was 1.0 mL·min^-1, injection volume was 20 μL, column temperature was 45 ℃. RESULTS The calibration curves of xylose and xylooligosaccharide in the mixed reference substances or sample solutions were well separated with good correlation coefficient (r=0.999 4-0.999 9) in the concentration range of 0.05-0.5 mg·mL^-1. The recoveries(n=6) of 7 components at high and low concentration were 90.6%-103.2%, the RSDs were <
4. 0%. The contents of xylooligosaccharide (X₂-X₇) in three batches were 313, 312, 310 mg·g^-1, and xylooligosaccharide (X₂-X₄) 282, 279, 278 mg·g^-1, respectively. CONCLUSION The method is simple, rapid, sensitive and reproducible, providing a more accurate and reliable method for the quality control and research of production process in xylooligosaccharide products.