Determination of Ganoderic Acid C₁ in Ganoderma Lucidum Spore Oil by HPLC

OBJECTIVE To establish a method for the content determination of ganoderic acid C₁ in Ganoderma lucidum spore oil. METHODS The determination was performed on an Agilent Zorbax-SB C₈ column(150 mm×4.6 mm, 3.5 μm). The mobile phase was acetonitrile-0.1% acetic acid(25:75) as a flow rate of 1 mL·min-1. The detection wavelength was 256 nm and the column temperature was 22℃. RESULTS The ganoderic acid C₁ showed a good linearity in the range of 1.002-25.04 µg·mL^-1 (r=0.999 9) and the average recovery was 97.47%. The contents of ganoderic acid C₁ in Ganoderma lucidum spore oil from different origins had a certain difference. The contents of ganoderic acid C₁ in samples were low and some samples didn't contain ganoderma lucidum acid C₁. CONCLUSION The established method is simple, accurate and repeatable, which can provide reference for the quality control of Ganoderma lucidum spore oil.