Study on HPLC Characteristic Spectrum of Traditional She Medicine–Shi Liang Cha and Determination of Four Active Components

OBJECTIVE To establish an HPLC characteristic spectrum of traditional SHE medicine–Shi Liang Cha and simultaneous determination of four active components. METHODS With rutin, kaempferol-3-O-rutinoside, quercetin and kaempferol as reference substance, the HPLC analysis was carried on an Agilent Zorbax SB-C₁₈ column (4.6 mm × 250 mm, 5 μm) with the column temperature of 30 ℃; the mobile phase was acetonitrile-0.1% phosphoric acid solution (gradient elution) at the flow rate of 1.0 mL·min^-1; the detection wavelength was 360 nm. The similarity evaluation system for chromatographic fingerprint of TCM was used to calculate the similar degree. RESULTS The HPLC characteristic spectrum of Shi Liang Cha was established with 5 common peaks. The rutin, kaempferol-3-O-rutinoside, quercetin and kaempferol showed a good linear relationship at the range of 4.525-452.8 μg·mL^-1(r=0.999 9), 8.096-809.6 μg·mL^-1(r=1.000 0), 0.654-85.15 μg·mL^-1(r= 1.000 0), 2.048-136.6 μg·mL^-1(r=1.000 0), respectively; the average recoveries were 100.51%(n=6, RSD=0.43%), 100.19%(n=6, RSD=0.88%), 99.98%(n=6, RSD=0.77%), 100.26%(n=6, RSD=0.69%), respectively.CONCLUSION With a good correlation, the characteristic spectrum can be combined with the assay of 4 components to control the quality of Shi Liang Cha comprehensively, and provide scientific basis for its rational use.