Simultaneous Determination of Four Saponins in Tengzhuweikang Granules by HPLC

OBJECTIVE To establish a method for simultaneous determination of four saponins(ginsenoside Re, ginsenoside Rb₁, ginsenoside Ro and chikusetsusaponin Ⅳa) in Tengzhuweikang granules by HPLC. METHODS The HPLC separation was achieved on a Hypersil GOLD C₁₈(250 mm×4.6 mm, 5 μm) column. The mobile phase consisted of acetonitrile-0.2% phosphoric acid solution with gradient elution(-5-0 min, 19% acetonitrile; 0-14 min, 19%→26% acetonitrile; 14-22 min, 26%→29% acetonitrile; 22-30 min, 29% acetonitrile; 30-40 min, 29%→35% acetonitrile; 40-55 min, 35% acetonitrile) at a flow rate of 1.0 mL·min^-1. The detection wavelength was set at 203 nm and the colum temperature was 30 ℃. RESULTS The linear ranges of ginsenoside Re, ginsenoside Rb₁, ginsenosideRo, chikusetsusaponin Ⅳa were 0.080 4-1.608 μg (r=1), 0.108 8-2.176 μg(r=0.999 9), 0.288 8-5.776 μg(r=0.999 9), 0.176 0-3.520 μg(r=0.999 9), respectively. The average recoveris(n=6) were 99.41%, 101.92%, 99.76%, 100.31%, respectively. RSD of the recoveris was 2.22%, 2.07%, 0.33%, 0.64%, respectively. CONCLUSION The method is simple and repeatable, can be used for the determination of ginsenoside Re, ginsenoside Rb₁, ginsenoside Ro and chikusetsusaponin Ⅳa in Tengzhuweikang Granules.