Application of lipase in the preparation of Pregabalin

OBJECTIVE To review and discuss on the application of lipase in pregabalin chemoenzymatic synthesis. METHODS The paper summarized the application of lipase in the synthesis of the chiral intermediates by reviewing the literatures, and the lipase of resolving 2-carboxyethyl-3-cyano-5-methylhexanoic acid ethyl ester(CNDE). RESULTS High selectivity, high yield, friendly environment on the lipase catalyzed resolution of pregabalin intermediate. CONCLUSION Compared with the chemical synthesis of pregabalin, lipase in preparation of pregabalin has obvious advantages in the process, it has a good application prospect.