Determination of Linarin and Apigenin in Cirsii Herba Dispensing Granule by HPLC

OBJECTIVE To develop a method for content determination of linarin and apigenin in Cirsii Herba Dispensing Granule by HPLC. METHODS The separation was carried out on Diamonsil-C₁₈ column (4.6 mm×250 mm,5 μm) under gradient elution with acetonitrile-1% glacial acetic as mobile phase at a flow rate of 1.0 ml·min^-1. The detection wavelength was 335 nm and the column temperature was maintained at 30℃. RESULTS There were good linear relationships between the masses and the peak areas of linarin and apigenin in the ranges of 0.3-1.5 μg (r=0.999 4) and 0.075-0.375 μg (r=0.999 7), respectively. The average recoveries were 99.19% and 99.12%, and RSDs were 1.27% and 1.26%. CONCLUSION The method is simple and reliable for the determination of linarin and apigenin in Cirsii Herba Dispensing Granule.