Organic Solvent-Based Dispersive Liquid Phase Microextraction Combined with HPLC for Determination of Schizandrin A and Schisantherin A in Schisandra chinensis (Turcz.) Baill.

OBJECTIVE To enrich and determine the schizandrin A and schisantherin A in Schisandra chinensis (Turcz.) Baill. by developing an organic solvent-based dispersive liquid phase microextraction combined with HPLC method.METHODS Under the optimal conditions(extraction solvent was CCl₄, the volume of extraction solvent was 60 μL, pH of donor phase was 1 and the extraction time was 4 min, centrifuge at 1 500 r·min^-1 for 2 min), the analytes were extracted. HPLC analyses were performed on a C₁₈ column. The mobile phase consisted of a mixture of methanol and phosphorous acid solution (69:31), and the detection wavelength was set at 254 nm.RESULTS The enrichment factors for schizandrin A and schisantherin A were 5 112 and 1 118, respectively. The linear range was both 0.10-19 μg·mL^-1. And the recoveries of analytes were 100.1% for schizandrin A and 94.4% for schitherin A, respectively.CONCLUSION The method is sensitive, reproductive, simple, rapid and environmental protection, and it can be used to determine schizandrin A and schisantherin A in Schisandra chinensis (Turcz.) Baill..