Fingerprint and Multi-Component Quantification Analysis of Xiaoyan Tablets by HPLC

OBJECTIVE To establish HPLC fingerprint of Xiaoyan tablets and simultaneously determine 5 chemical compositions (aesculetin, baicalin, linarin, baicalein, wogonin), provide evidence for quality control.METHODS The analytical column was ACE C₁₈(4.6 mm×250 mm, 5 μmm). The mobile phase was acetonitrile-water(containing 0.1% formic acid) with gradient elution, and the detection wavelength was set at 321 nm. "Chromatographic fingerprint similarity evaluation software" (version 2004 A) was used to evaluate similarity for the 11 batches Xiaoyan tablets.RESULTS The similarity among the 11 batches was all >0.90, and 25 common peaks were defined in the HPLC fingerprint, and 5 characteristic peaks were earmarked 5 characteristic peaks (aesculetin, baicalin, linarin, baicalein, wogonin) were earmarked and determined.CONCLUSION The HPLC fingerprint method is high characteristics, and the determination of multi-index components is repeatability, accurate and simple, which can be used efficiently in the quality control composite evaluation of Xiaoyan tablets.