Comparison of the Results of in Vitro 3T3 NRU Phototoxicity Test Between Different Light Sources

OBJECTIVE To compare the results of six kinds of reference materials under two different light sources irradiation in vitro 3T3 phototoxicity test. METHODS According to the guideline of OECD 432, carried out the in vitro 3T3 NRU phototoxicity test irradiated by simulated sunlight and ultraviolet light source separately. RESULTS The results of the photo irritation factor(PIF) and the mean photo effect(MPE) of six kinds of reference materials under different light sources were close to the reference value listed in the OECD 432, and the IC₅₀ values for each substance were also similar. CONCLUSION After two light sources irradiation, there is no difference among six kinds of reference materials on in vitro 3T3 phototoxicity test results.