Effect and Mechanism of Coptisine on the Rat Model of Chronic Renal Failure

OBJECTIVE To explore the effect and mechanism of coptisine on the rat model of chronic renal failure(CRF). METHODS The 60 male SD rats were divided into 6 groups:the blank group, model group, positive control group and the 200,100 and 50 mg·kg^-1 dose of coptisine groups. Rats were gavaged adenine 28 d, except the blank group. The high, middle, low dose of coptisine group rats were gavaged 200, 100 and 50 mg·kg^-1 coptisine each day. After therapy, weight, urinary production and urinary protein in 24 h was detected, urea nitrogen(BUN), creatinine(SCr), TGF-β₁ and PAI-1 in serum was detected by Elisa; the expression of TGF-β₁ and PAI-1 in kidney tissue was detected by Western blot. RESULTS Compared with model group, in 200, 100 and 50 mg·kg^-1 dose of coptisine groups, weight and urinary production of 24 h were increased(P<0.05) and urinary protein in 24 h was decreased(P<0.05); BUN, SCr, TGF-β₁ and PAI-1 level in serum and the expression of TGF-β₁ and PAI-1 protein was decreased(P<0.05). CONCLUSION Coptisine has a certain effect on the renal funtion of CRF rats and its mechanism is related to the expression of TGF-β₁ and PAI-1 in srtum and renal tissue.