Effect of Phyllodium Pulchellum Alkaloid on the Proliferation and Extracellular Matrix in Human Hepatic Stellate Cell Stimulated by Acetaldehyde

OBJECTIVE To investigate the effects of Phyllodium puchellum alkaloid on the proliferation and extracellular matrix in human hepatic stellate cell (HSC-LX2) stimulated by acetaldehyde. METHODS MTT assay was used to evaluate the inhibitory effect of the drugs on HSC-LX2. The content of LDH was determined by using colorimetric method, Real-time PCR was applied to measure the expression of α-SMA, Co1-Ⅰ, MMP-2 and TIMP-1 mRNA. RESULTS P. pulchellum alkaloid could inhibit proliferation of HSC-LX2 cells in concentration manners. The activity of LDH in supernatant had no statistical significance compared with control group. From the results of Real-time PCR, the expression of α-SMA, CoL-I and TIMP-1 mRNA in drug groups were notably lower than those in model group stimulated by acetaldehyde, while the MMP-2 mRNA was increased. CONCLUSION The P. pulchellum alkaloid can inhibit the expression of α-SMA, Col-I and TIMP-1 mRNA in HSC-T6, reduce the synthesis and deposition of extracellular matrix in liver, so as to interfere with course of the liver fibrosis.