Determination of Hesperidin in Bohol Pill by ASE-HPLC

OBJECTIVE To develop a method for the determination of hesperidin in Bohol pill by accelerated solvent extraction(ASE)-HPLC method. METHODS Hesperidin in Bohol pill was extracted by ASE. The analytical column was Kinetex XB-C₁₈(100 mm×4.60 mm, 2.6 mm). The mobile phase was methanol-7% acetic acid(37:63) with flow rate 0.7 mL·min^-1. The column temperature was 40℃. The detector wavelength was set at 283 nm. RESULTS The linear range was 3.707-74.140 μg·mL^-1. The average recovery of hesperidin was 99.33%(RSD=0.2%). CONCLUSION The established method is simple, rapid and reliable, which can be applied to determine hesperidin in Bohol pill.