Study of Screening the Effective Part of Alcohol Extract of Artemisia Anomala S. Moore on Anti Focal Cerebral Ischemia

OBJECTIVE To screen the effective part of alcohol extract of Artemisia anomala S. Moore on anti focal cerebral ischemia. METHODS ICR mice were randomly divided into sham operation group, model group, nimodipine group (10 mg·kg^-1), petroleum ether extraction (part Ⅰ), ethyl acetate (part Ⅱ), n-butanol extract (part Ⅲ) and the remaining aqueous layer (part Ⅳ) of alcohol extract of Artemisia anomala S. Moore, parts Ⅰ~Ⅳ were divided into 3.25, 6.50 g·kg^-1 dose groups, continuous administration 7 d before modeling. The improved Zea Longa method was used to establish animal model of focal cerebral ischemia. After 6 h, the nerve function score, brain index, brain water content, infarct volume and pathological changes of brain tissue of mice were measured; 3.25, 6.50 g·kg^-1 dose groups of the effective part of alcohol extract of Artemisia Anomala S. Moore measured superoxide dismutase activity and malondialdehyde content in brain tissue by useing xanthine oxidase and thiobarbituric acid method. RESULTS Compared with the model group, part Ⅰ, part Ⅱ and part Ⅲcould improve brain edema caused by focal cerebral ischemia in different degrees, and the part Ⅲ could significantly reduce nerve function score, brain index, brain water content and infarct volume in mice (P<0.01 or P<0.05). Pathological examination showed that compared with the model group, the part Ⅲ could significantly improve the degree of brain neuron nucleus dissolution and the extent of irregular nucleus(P<0.01). Compared with the model group, SOD activity of 3.25, 6.50 g·kg^-1 dose groups of the part Ⅲ in mice brain tissue significantly increased (P<0.01 or P<0.05), MDA levels significantly decreased (P<0.05). CONCLUSION The main active part of alcohol extract of Artemisia anomala S. Moore on anti focal cerebral ischemia is n-butanol extract (part Ⅲ) after preliminary screening and its mechanism may be related with its antioxidant effects.