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引用本文:罗洁,范旭航,崔思娇,石绍淮,毕开顺,贾英.知母的UPLC指纹图谱及聚类分析[J].中国现代应用药学,2013,30(1):28-31.
LUO Jie,FAN Xuhang,CUI Sijiao,SHI Shaohuai,BI Kaishun,JIA Ying.UPLC Fingerprint of Anemarrhenae Rhizoma and Its Hierarchical Cluster Analysis[J].Chin J Mod Appl Pharm(中国现代应用药学),2013,30(1):28-31.
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知母的UPLC指纹图谱及聚类分析
罗洁1, 范旭航1, 崔思娇1, 石绍淮1, 毕开顺2, 贾英1
1.沈阳药科大学,中药学院,沈阳 110016;2.沈阳药科大学,药学院,沈阳 110016
摘要:
目的 建立知母的超高效液相指纹图谱。方法 采用ACQUITY UPLC HSS T3(2.1 mm×100 mm,1.8 μm)色谱柱;流动相为乙腈-0.03%磷酸,梯度洗脱,流速为0.5 mL·min-1,柱温为30 ℃,检测波长为210 nm。结果 建立了知母的UPLC指纹图谱共有模式,标定了12个共有峰,并指认了6个主要色谱峰,各色谱峰有较好的分离。根据聚类分析结果,可将所收集的知母样品分为两类。结论 本方法快速、高效,可用于知母的质量评价。
关键词:  知母  指纹图谱  超高效液相色谱法  聚类分析
DOI:
分类号:
基金项目:辽宁省科学技术计划项目(2011412004);辽宁省教育厅创新团队项目(2009T097)
UPLC Fingerprint of Anemarrhenae Rhizoma and Its Hierarchical Cluster Analysis
LUO Jie1, FAN Xuhang1, CUI Sijiao1, SHI Shaohuai1, BI Kaishun2, JIA Ying1
1.Shenyang Pharmaceutical University, School of Traditional Chinese Materia Medica, Shenyang 110016, China;2.Shenyang Pharmaceutical University, School of Pharmacy, Shenyang 110016, China
Abstract:
OBJECTIVE To establish fingerprint of Anemarrhenae Rhizoma UPLC. METHODS The UPLC fingerprint of Anemarrhenae Rhizoma were determined on an HSS T3 column (2.1 mm×100 mm,1.8 μm) eluted with the mobile phase consisted of acetonitrile and 0.03% phosphoric acid in gradient mode; flow rate: 0.5 mL·min-1; column temperature: 30 ℃ and the detection wavelength was set at 210 nm. RESULTS The common mode of the UPLC fingerprint was set up under the established condition. There were 12 common peaks in the fingerprint of 16 samples, six of which were identified. The Anemarrhenae Rhizoma from 16 different areas could be divided into 2 grades through the results of hierarchical cluster analysis. CONCLUSION The method was fast and accurate. The chromatographic profile of Anemarrhenae Rhizoma with high specificity can be used to control the quality of Anemarrhenae Rhizoma.
Key words:  Anemarrhenae Rhizoma  fingerprint  UPLC  hierarchical cluster analysis
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