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引用本文:唐洪梅1,李得堂2,闫雪2,丘振文1,蔡庆群1.HPLC同时测定疗筋涂膜剂中5种大黄成分的含量[J].中国现代应用药学,2009,(5):408-411.
TANG Hongmei1, LI Detang2, YAN Xue2, QIU Zhenwen1, CAI Qingqun1.Simultaneous Determination 5 Contents of the Rhubarb in the Liaojin Film by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2009,(5):408-411.
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HPLC同时测定疗筋涂膜剂中5种大黄成分的含量
唐洪梅1,李得堂2,闫雪2,丘振文1,蔡庆群1
作者单位
唐洪梅1,李得堂2,闫雪2,丘振文1,蔡庆群1  
摘要:
目的建立高效液相色谱法同时测定疗筋涂膜剂中5种成分的含量。方法采用Hypersil ODS-C18柱(250 mm × 4.6 mm,5 μm) , 甲醇-0.05%磷酸(85∶15)为流动相,流速:0.8 mL·min-1,柱温:34 ℃,检测波长:254 nm。结果芦荟大黄素在9.11~145.76 μg·mL-1(r=0.999 4),大黄酸在5.63~90.08 μg·mL-1(r=0.999 3),大黄素在5.44~87.04 μg·mL-1(r=0.999 9),大黄酚在6.13~98.08 μg·mL-1(r=0.999 9),大黄素甲醚在2.72~43.52 μg·mL-1 (r=0.999 8)线性良好;平均回收率分别为97.64%、98.14%、99.52%、99.36%、98.43%;RSD分别为1.29%、1.81%、1.40%、0.59%、1.58%。结论该方法简便、可靠、准确,可用于疗筋涂膜剂的质量控制。
关键词:  疗筋涂膜剂  质量控制  高效液相色谱法
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Simultaneous Determination 5 Contents of the Rhubarb in the Liaojin Film by HPLC
TANG Hongmei1, LI Detang2, YAN Xue2, QIU Zhenwen1, CAI Qingqun1
Abstract:
OBJECTIVE To establish an HPLC method for the determination of Chrysophanol, Emodin, Physcion, Aloeemodin and in Liaojin film.METHODS The determination was performed on Hypersil ODS-C18 column(250 mm x 4.6 mm,5 μm)with mobile phase consisted of methanol-0.05%phosphoric acid (85∶15)at a flow rate of 0.8 mL·min-1.The UV detection wavelength was set at 254 nm.RESULTS The calibration curves were linear in the range of 9.11-145.76 μg·mL-1(r=0.999 4)for Aloeemodin,5.63-90.08 μg·mL-1(r=0.999 3)for Rhein,of 5.44-87.04 μg·mL-1(r=0.999 9)for Emodin,6.13-98.08 μg·mL-1 (r=0.999 9)for Chrysophanol and 2.72-43.52 μg﹒mL-1(r=0.999 8) for Physcion. The average recovery was 97.64%,98.14%,99.52%,99.36%,98.43%;RSD was1.29%,1.81%,1.40%,0.59%,1.58%.CONCLUSION The determination method is simple,reliable,accurate, and it can be used for quality control of Liaojin film.
Key words:  Liaojin film  quality control  HPLC
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