| 摘要: |
| 目的 建立转化糖注射液中果糖、葡萄糖的含量测定方法。方法 色谱柱为Hamilton HC-75H(305 mm×7.8 mm，9 μm)，流动相为0.04 mol·L-1磷酸，流速为0.5 mL·min-1，检测波长为200 nm，柱温为50 ℃。结果 果糖和葡萄糖的检测限分别为0.28，0.52 μg；线性范围分别为1.27~3.81 mg·mL-1(r=0.999 4)、1.26~3.76 mg·mL-1(r=0.999 2)；加样回收率分别为100.8%，100.4%。结论 本法适用于转化糖注射液中果糖、葡萄糖的含量测定。 |
| 关键词: 转化糖注射液 高效液相色谱法 果糖 葡萄糖 含量测定 |
| DOI： |
| 分类号: |
| 基金项目: |
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| Determination of Fructose and Glucose in Invert Sugar Injection by HPLC |
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ZHOU Na
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Hangzhou Minsheng Pharmaceutical Co., LTD, Hangzhou 310011, China
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| Abstract: |
| OBJECTIVE To establish a method for the determination of fructose and glucose in invert sugar injection. METHODS Hamilton HC-75H(305 mm×7.8 mm, 9 μm) was used. The mobile phase was 0.04 mol·L-1 phosphoric acid at a flow rate of 0.5 mL·min-1. The determination wavelength was 200 nm. The temperature of the column was 50 ℃. RESULTS To fructose and glucose, the limit of detection was 0.28 μg and 0.52 μg, the linear range was 1.27-3.81 mg·mL-1(r=0.999 4) and 1.26-3.76 mg·mL-1(r=0.999 2), the spiked recovery was 100.8% and 100.4%, respectively. CONCLUSION The method is suitable for the determination of fructose and glucose in invert sugar injection. |
| Key words: invert sugar injection HPLC fructose glucose content determination |
