| 摘要: |
| 生物标志物是目前临床上用于疾病的早期诊断、分类、预后评价的重要指标。近年来，生物标志物检测方法迅猛发展，在抗原抗体结合原理检测方法的基础上，结合光、电等信号变化，使检测方法的特异性及灵敏度大大提高；同时在识别元件方面发展了分子印迹、核酸适配体、单域抗体等方法，弥补了传统抗体稳定性、重复性差等不足。本文对目前的生物标志物检测方法做一综述，并对其未来的发展进行了展望。 |
| 关键词: 生物标志物 检测方法 早期诊断 |
| DOI：10.13748/j.cnki.issn1007-7693.2020.03.025 |
| 分类号: |
| 基金项目:广东省科技计划（2016A010103016）；广州市科技计划（201607010148） |
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| Research Progress of New Methods for Biomarker Detection |
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LIU Ruixuan, ZHU Quanhong
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College of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China
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| Abstract: |
| Biomarker is an important indicator for clinical diagnosis, classification, and prognosis of diseases. In recent years, biomarker detection methods have been developed rapidly. On the basis of the antigen-antibody binding principle, the traditional detection methods have been improved combined with the changes of light and electricity and the specificity and sensitivity have been increased satisfactorily. In the field of recognition element, molecular imprinting, aptamer and single-domain antibodies have been developed for improving the stability and reproducibility of detection methods. This article will review the current biomarker detection methods and prospects for their future development. |
| Key words: biomarker detection methods early diagnosis |
