| 引用本文: | 梁丽娜,张晓千,李省,张智强.1H核磁共振定量法测定氢溴酸右美沙芬片含量[J].中国现代应用药学,2020,37(3):304-307. |
| LIANG Lina,ZHANG Xiaoqian,LI Sheng,ZHANG Zhiqiang.Determination of Content of Dextromethorphan Hydrobromide Tablets Based on Quantitative Proton Nuclear Magnetic Resonance[J].Chin J Mod Appl Pharm(中国现代应用药学),2020,37(3):304-307. |
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| 摘要: |
| 目的 采用1H核磁共振定量法（quantitative proton nuclear magnetic resonance，qHNMR）测定氢溴酸右美沙芬片中氢溴酸右美沙芬含量。方法 qHNMR以氢溴酸右美沙芬化学位移δ 7.05~7.15 ppm处质子峰为定量峰，苯甲酸苄酯δ 5.38 ppm处为内标峰，在温度为300 K，采样时间为4.01 s，弛豫延迟时间10 s，扫描次数为32次条件下采集混合物qHNMR图谱。比较qHNMR与HPLC的含量测定结果。结果 qHNMR专属性较高，线性范围5.88~23.08 mg·mL-1，回收率为98.4%~99.7%，定量限为0.45 mg·mL-1，检测限为0.10 mg·mL-1。qHNMR含量测定结果与HPLC的测定结果基本一致。结论 qHNMR可用于氢溴酸右美沙芬片中氢溴酸右美沙芬的含量测定。 |
| 关键词: 氢溴酸右美沙芬片 苯甲酸苄酯 1H核磁共振定量法 |
| DOI：10.13748/j.cnki.issn1007-7693.2020.03.009 |
| 分类号:R917 |
| 基金项目:河南省自然科学基金项目（162300510118） |
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| Determination of Content of Dextromethorphan Hydrobromide Tablets Based on Quantitative Proton Nuclear Magnetic Resonance |
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LIANG Lina1, ZHANG Xiaoqian1, LI Sheng1, ZHANG Zhiqiang2
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1.Zhengzhou Shuqing Medical College, Zhengzhou 450064, China;2.Tianjin Institute of Pharmaceutical Research Pharmaceutical Co., Ltd., Tianjin 300301, China
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| Abstract: |
| OBJECTIVE to determine the content of dextromethorphan hydrobromide tablets based on quantitative proton nuclear magnetic resonance(qHNMR). METHODS Proton signal peaks at δ 7.05-7.15 ppm of dextromethorphan hydrobromide and δ 5.38 ppm of benzyl benzoate were served as quantitative peaks and internal standard peaks, respectively. The spectra of the mixture were collected at 300 K with 4.01 s acquisition time, 10 s relaxation delay and 32 scanning times. The results of qHNMR and HPLC were also been compared. RESULTS The speciality of qHNMR was very high. There was a linear relationship for dextromethorphan hydrobromide in the range of 5.88-23.08 mg·mL-1. The recoveries were in the range of 98.4%-99.7%. The limit of quantitation was 0.45 mg·mL-1 and the limit of detection was 0.10 mg·mL-1. The content determination results of qHNMR were generally consistent with those of HPLC. CONCLUSION The qHNMR can be used for the quantitative determination of dextromethorphan hydrobromide tablets. |
| Key words: dextromethorphan hydrobromide tablets benzyl benzoate quantitative proton nuclear magnetic resonance |
