| 引用本文: | 陈雪娟,安方玉,颜春鲁,刘雪松,王彦博,靳安顺,成新平,杨晓蓉.玫瑰红芪当归水煎剂对围绝经期大鼠IL-2含量及Sparc表达的影响[J].中国现代应用药学,2020,37(2):149-154. |
| CHEN Xuejuan,AN Fangyu,YAN Chunlu,LIU Xuesong,WANG Yanbo,JIN Anshun,CHENG Xinping,YANG Xiaorong.Effect of Rose, Astragalus and Angelica Decoction on IL-2 Contents and Sparc Expression in Perimenopausal Rats[J].Chin J Mod Appl Pharm(中国现代应用药学),2020,37(2):149-154. |
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| 玫瑰红芪当归水煎剂对围绝经期大鼠IL-2含量及Sparc表达的影响 |
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陈雪娟1, 安方玉2, 颜春鲁3, 刘雪松1, 王彦博3, 靳安顺4, 成新平4, 杨晓蓉5
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1.甘肃中医药大学基础医学院, 兰州 730000;2.甘肃中医药大学教学实验实训中心, 兰州 730000;3.甘肃中医药大学中西医结合学院, 兰州 730000;4.甘肃中医药大学公共卫生学院, 兰州 730000;5.甘肃中医药大学临床医学院, 兰州 730000
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| 摘要: |
| 目的 观察玫瑰红芪当归水煎剂对围绝经期大鼠IL-2含量及Sparc表达变化的影响。方法 通过手术切除Wistar ♀大鼠双侧卵巢复制围绝经期综合征模型。造模后第7天用ELISA法检测血清雌二醇（estradiol，E2）含量确定造模是否成功，于造模成功后第2周将切除卵巢的大鼠随机分为模型组，玫瑰红芪当归水煎剂低、中、高剂量组（1.4，2.8，5.6 g·kg-1）和戊酸雌二醇片组（0.09 mg·kg-1），每组10只。另设10只Wistar ♀大鼠作为正常组。末次给药24 h后，腹主动脉采血处死大鼠后，称取各组大鼠体质量，并计算其子宫指数及胸脾指数，HE染色法观察各组大鼠子宫组织形态学变化，ELISA法测定各组大鼠血清E2和白细胞介素-2（interleukin-2，IL-2）的含量，实时荧光定量PCR法检测各组大鼠富含半胱氨酸的酸性分泌蛋白（secreted protein acidic and rich in cysteine，Sparc）的基因表达，免疫组化法观察各组大鼠雌激素受体α（estradiol receptor α，ERα）的蛋白表达。结果 与模型组相比，玫瑰红芪当归水煎剂中、高剂量组和戊酸雌二醇片组能明显降低大鼠的体质量，明显升高大鼠的子宫指数、胸腺指数、脾脏指数、Sparc的基因表达和ERα的蛋白表达（P<0.05）；玫瑰红芪当归水煎剂各治疗组和戊酸雌二醇片组能明显升高模型组大鼠E2含量和IL-2的含量（P<0.05）。HE染色发现，玫瑰红芪当归水煎剂高剂量组和戊酸雌二醇片组子宫黏膜层出现腺体，单层柱状上皮细胞排列整齐。结论 玫瑰红芪当归水煎剂治疗围绝经期综合征与调节机体内分泌-免疫系统有关，为临床应用和新药开发提供实验依据。 |
| 关键词: 玫瑰红芪当归水煎剂 围绝经期 内分泌激素 免疫功能 富含半胱氨酸的酸性分泌蛋白 |
| DOI：10.13748/j.cnki.issn1007-7693.2020.02.005 |
| 分类号:R285.5 |
| 基金项目:甘肃省自然科学基金项目（1308RJZA161）；甘肃省高校大学生就业创业能力提升工程项目（6-1）；甘肃中医药大学教学研究与教学改革项目（ZH-201606） |
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| Effect of Rose, Astragalus and Angelica Decoction on IL-2 Contents and Sparc Expression in Perimenopausal Rats |
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CHEN Xuejuan1, AN Fangyu2, YAN Chunlu3, LIU Xuesong1, WANG Yanbo3, JIN Anshun4, CHENG Xinping4, YANG Xiaorong5
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1.Gansu University of Traditional Chinese Medicine, Basic Medical College, Lanzhou 730000, China;2.Gansu University of Traditional Chinese Medicine, Teaching Experiment Training Center, Lanzhou 730000, China;3.Gansu University of Traditional Chinese Medicine, Clinical College of Integrated Traditional Chinese and Western Medicine, Lanzhou 730000, China;4.Gansu University of Traditional Chinese Medicine, School of Public Health, Lanzhou 730000, China;5.Gansu University of Traditional Chinese Medicine, Clinical College, Lanzhou 730000, China
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| Abstract: |
| OBJECTIVE To observe the effect of rose, astragalus and angelica decoction on the changes of IL-2 and secreted protein acidic and rich in cysteine(Sprac) expression during perimenopause period in rats. METHODS Peri-menopausal syndrome models were established by surgical removal of the bilateral ovaries of female Wistar rats. After successful modeling by detecting the content of estradiol(E2) by ELISA on the 7th postoperative day, the rats were randomly divided into model group, rose, astragalus and angelica decoction low, medium and high dose groups(1.4, 2.8, 5.6 g·kg-1) and estradiol valerate tablet group(0.09 mg·kg-1) in the second week after successful modeling, each for 10 rats. Another 10 female Wistar rats were picked as the normal group. 24 h after the last administration, abdominal aortic blood was took to measure the indexes of the uterus, thymus and spleen was checked by weighing method, HE staining to observe uterus tissue morphology, the concentration of E2 and IL-2 in serum was measured by ELISA, Sparc gene expression of uterus tissue was checked by real-time fluorescence quantitative PCR, estradiol receptor α(ERα) protein expression of uterus tissue was determined by immunohistochemistry. RESULTS Compared with the model group, the weight of rats in rose, astragalus and angelica decoction middle, high dose group and estradiol valerate tablet group was significantly lower, the index of uterus, thymus and spleen were significantly higher, the gene expression of Sparc was raised and the protein of ERα were also significantly higher(P<0.05); serum E2 and IL-2 in rose, astragalus and angelica decoction treatment group and estradiol valerate tablet group were significantly higher(P<0.05). The glands appeared and monolayer columnar epithelial cells arranged neatly in high dose group of astragalus and angelica decoction treatment group and estradiol valerate tablet group by HE staining. CONCLUSION Rose, astragalus and angelica decoction can regulate beneficially the unbalanced reproductive endocrine-immune net-work. It can provide experimental basis for clinical application and new perimenopause drugs development. |
| Key words: rose,astragalus and angelica decoction perimenopause endocrine hormones immunologic function secreted protein acidic and rich in cysteine |
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