| 引用本文: | 廖晓燕,洪圆,姜雯文,梁淑彩.HPLC-荧光法测定酒女贞子中羟基酪醇、红景天苷和酪醇[J].中国现代应用药学,2019,36(24):3064-3067. |
| LIAO Xiaoyan,HONG Yuan,JIANG Wenwen,LIANG Shucai.Determination of Hydroxytyrosol, Salidroside and Tyrosol in Wine-fried Ligustri Lucidi Fructus by HPLC with Fluorescence Detection[J].Chin J Mod Appl Pharm(中国现代应用药学),2019,36(24):3064-3067. |
|
| 摘要: |
| 目的 建立一种简单、高灵敏度，可同时测定酒女贞子中羟基酪醇、红景天苷和酪醇含量的HPLC-荧光法。方法 采用Amethyst C18-P柱（250 mm×4.6 mm，5 μm），以水-乙腈（92∶8）为流动相等度洗脱，激发波长为280 nm，发射波长为310 nm，柱温35℃。结果 羟基酪醇、红景天苷和酪醇在一定浓度范围内线性关系良好，检测限分别可达23.0，10.5，9.82 ng·mL-1。酒女贞子中羟基酪醇、红景天苷和酪醇的含量范围分别为642.9~867.2 μg·g-1，1 932.3~15 357.5 μg·g-1，239.1~638.1 μg·g-1。结论 所建立的HPLC-荧光法灵敏度高，选择性好、准确度高，可用于酒女贞子中羟基酪醇、红景天苷和酪醇的含量测定。 |
| 关键词: 高效液相色谱法 荧光 羟基酪醇 红景天苷 酪醇 酒女贞子 |
| DOI：10.13748/j.cnki.issn1007-7693.2019.24.012 |
| 分类号:R917.101 |
| 基金项目:武汉大学教学改革研究项目（JG201670，2018JG031） |
|
| Determination of Hydroxytyrosol, Salidroside and Tyrosol in Wine-fried Ligustri Lucidi Fructus by HPLC with Fluorescence Detection |
|
LIAO Xiaoyan, HONG Yuan, JIANG Wenwen, LIANG Shucai
|
|
Wuhan University School of Pharmaceutical Sciences, Wuhan 430071, China
|
| Abstract: |
| OBJECTIVE To develop a simple and sensitive HPLC with fluorescence detection method for simultaneous determination of hydroxytyrosol, salidroside and tyrosol in wine-fried Ligustri Lucidi Fructus. METHODS The analysis was performed on a Amethyst C18-P column(250 mm×4.6 mm, 5 μm) with isometric elution using water and acetonitrile(92:8). The detection wavelengths were at 280 nm and 310 nm for excitation and emission. The column temperature was 35℃. RESULTS The good linearity was in the certain concentration range, and the limit of quantitation of hydroxytyrosol, salidroside and tyrosol were 23.0, 10.5, 9.82 ng·mL-1, respectively. The range of content of hydroxytyrosol, salidroside and tyrosol in wine-fried Ligustri Lucidi Fructus were 642.9-867.2 μg·g-1, 1 932.3-15 357.5 μg·g-1, 239.1-638.1 μg·g-1, respectively. CONCLUSION The developed HPLC-Fluorescence detection method is sensitive, selective and accurate for simultaneous determination of hydroxytyrosol, salidroside and tyrosol in wine-fried Ligustri Lucidi Fructus. |
| Key words: HPLC Fluorescence detection hydroxytyrosol salidroside tyrosol wine-fried Ligustri Lucidi Fructus |
