| 摘要: |
| 目的 建立牛黄清感胶囊HPLC指纹图谱，并测定其中7种有效成分的含量。方法 采用HPLC，色谱柱为Agilent SB-C18（250 mm×4.6 mm，5 μm），以乙腈（A）-0.1%磷酸水溶液（B）为流动相进行梯度洗脱；检测波长210 nm；柱温30℃。采用"中药色谱指纹图谱相似度评价系统（2004 A版）"进行相似度分析，并对指认的7个指标成分进行定量测定研究。结果 在特征图谱研究中，共确定牛黄清感胶囊HPLC指纹图谱22个共有峰，通过与对照品比较指认其中7个共有峰分别为绿原酸、咖啡酸、木犀草苷、黄芩苷、黄芩素、汉黄芩苷和汉黄芩素，利用相似度软件对12批样品指纹图谱进行分析，各批样品相似度均>0.90。定量分析条件通过方法学验证，平均加样回收率为99.1%~104.8%，RSD为1.30%~1.88%。结论 所建立的HPLC指纹图谱和含量测定分析方法可用于牛黄清感胶囊质量控制。 |
| 关键词: 牛黄清感胶囊 绿原酸 咖啡酸 高效液相色谱法 指纹图谱 相似度分析 |
| DOI：10.13748/j.cnki.issn1007-7693.2020.01.009 |
| 分类号:R917.101 |
| 基金项目: |
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| Study on Fingerprint and Multi-components Determination of Niuhuang Qinggan Capsules by HPLC |
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ZHAN Lili
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Department of Drug Administration, The Second Hospital of Shandong University, Jinan 250000, China
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| Abstract: |
| OBJECTIVE To establish the HPLC fingerprint and determine the contents of seven compounds in Niuhuang Qinggan capsules simultaneously. METHODS The separation was carried out on an Agilent SB-C18 column(250 mm×4.6 mm, 5 μm). The mobile phase was acetonitrile(A)-0.1% acetic acid solution(B) with gradient elution, the detection wavelength was 210 nm, and column temperature was 30℃. "Similarity evaluation system for chromatographic fingerprint of TCM (2004A)" was used to conduct, and the seven markers were quantified. RESULTS The common mode of the fingerprint showed 22 common peaks, 7 of which were identified by comparison with the reference substances. They were chlorogenic acid, caffeic acid, luteolin-7-o-glucoside, baicalin, baicalein, wogonoside and wogonin. The similar degrees of 12 batches of samples were > 0.90, and the average recoveries varied from 99.1%-104.8% with RSDs of 1.30%-1.88%. CONCLUSION The established method can be used for the quality control of Niuhuang Qinggan capsules. |
| Key words: Niuhuang Qinggan capsules chlorogenic acid caffeic acid HPLC fingerprint similarity evaluation |
