| 引用本文: | 许平翠,寿旦,吴人杰,李功华,王娜妮.白术挥发油GC-MS指纹图谱与抗氧化活性的谱效关系研究[J].中国现代应用药学,2019,36(11):1322-1327. |
| XU Pingcui,SHOU Dan,WU Renjie,LI Gonghua,WANG Nani.Study on the Spectrum-effect Relationship between GC-MS Fingerprint and Antioxidant Activity of Volatile Oil from Atractylodis Macrocephalae Rhizoma[J].Chin J Mod Appl Pharm(中国现代应用药学),2019,36(11):1322-1327. |
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| 白术挥发油GC-MS指纹图谱与抗氧化活性的谱效关系研究 |
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许平翠1,2, 寿旦1,2, 吴人杰1, 李功华3, 王娜妮1,2
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1.浙江省中医药研究院中药研究中心, 杭州 310007;2.浙江中医药大学药学院, 杭州 310053;3.浙江省立同德医院药学部, 杭州 310012
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| 摘要: |
| 目的 研究白术挥发油指纹图谱与抗氧化活性的谱效关系，筛选主要抗氧化活性成分。方法 采用GC-MS测定不同产地的15批白术饮片挥发油指纹图谱，通过清除1，1-二苯基-2-苦腈基（DPPH）和Fe3+还原/抗氧化能力法测定白术挥发油的抗氧化能力，运用偏最小二乘回归法进行数据分析，研究白术挥发油指纹图谱和抗氧化活性的相关性，并筛选活性色谱峰。结果 28个匹配指纹峰中，对挥发油清除DPPH自由基活性贡献度最大的前5个峰依次为：峰11 > 峰25 > 峰2 > 峰4 > 峰24；对挥发油还原Fe3+能力贡献度最大的前5个峰依次为：峰11 > 峰8 > 峰14 > 峰19 > 峰26。其中峰11的峰面积与挥发油清除DPPH自由基能力和还原Fe3+能力均呈正相关。通过与对照品比对确定峰11为苍术酮，并具有显著的抗氧化活性。结论 苍术酮不仅是白术挥发油的主要成分，还是主要的抗氧化活性物质。 |
| 关键词: 白术 挥发油 抗氧化活性 偏最小二乘回归法 谱效关系 |
| DOI：10.13748/j.cnki.issn1007-7693.2019.11.002 |
| 分类号:R284.1 |
| 基金项目:国家自然科学基金项目（81603252）；国家中药标准化项目（ZYBZH-Y-ZY-45）；浙江省自然科学基金项目（LQ17H280002）；浙江省中医药科技计划项目（2018ZY003，2016ZB008） |
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| Study on the Spectrum-effect Relationship between GC-MS Fingerprint and Antioxidant Activity of Volatile Oil from Atractylodis Macrocephalae Rhizoma |
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XU Pingcui1,2, SHOU Dan1,2, WU Renjie1, LI Gonghua3, WANG Nani1,2
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1.Chinese Medicine Research Center, Zhejiang Academy of Traditional Chinese Medicine, Hangzhou 310007, China;2.College of Pharmacy, Zhejiang Chinese Medical University, Hangzhou 310053, China;3.Department of Pharmacy, Tongde Hospital of Zhejiang Province, Hangzhou 310012, China
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| Abstract: |
| OBJECTIVE To study the spectrum-effect relationship between fingerprint and antioxidant activity of volatile oil from Atractylodis Macrocephalae Rhizoma, and to screen the main antioxidant active ingredients. METHODS GC-MS was used to determine the fingerprint of volatile oil from 15 batches of Atractylodis Macrocephalae Rhizoma from different producing areas. The antioxidant property of volatile oil was determined by removing 1,1-diphenyl-2-picrylonitrile(DPPH) and Fe3+ reduction/anti-resistance method. The data were analyzed by partial least squares regression to study the correlation between the fingerprint and antioxidant activity of volatile oil. The active chromatographic peaks were then screened. RESULTS Among the 28 matching fingerprint peaks, the top 5 chromatographic peaks that significantly positively related to scavenge DPPH free radicals were as follow:peak 11 > peak 25 > peak 2 > peak 4 > peak 24. The top 5 chromatographic peaks that significantly positively related to restore Fe3+ activity were as follow:peak 11 > peak 8 > peak 14 > peak 19 > peak 26. Peak area of peak 11 was positively correlated with both scavenge DPPH free radicals and restore Fe3+ activity. By comparing with the reference substance, it was confirmed that the peak 11 was atractylon. The results showed that atractyrone had significant antioxidant activity. CONCLUSION Atractylon is not only the main component of Atractylodis Macrocephalae Rhizoma volatile oil, but also the main antioxidant active substance. |
| Key words: Atractylodis Macrocephalae Rhizoma volatile oil antioxidant activity partial least squares regression spectral effect relationship |
