| 引用本文: | 赵维良,程夏倩,严爱娟,黄琴伟,徐靖,张文婷.灵芝孢子油指纹图谱研究及其混淆品鉴别[J].中国现代应用药学,2017,34(7):1011-1014. |
| ZHAO Weiliang,CHENG Xiaqian,YAN Aijuan,HUANG Qinwei,XU Jing,ZHANG Wenting.Studies on Fingerprint of Oil of Ganoderma Lucidum Spore and Identification for It's Confusion[J].Chin J Mod Appl Pharm(中国现代应用药学),2017,34(7):1011-1014. |
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| 灵芝孢子油指纹图谱研究及其混淆品鉴别 |
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赵维良1, 程夏倩2, 严爱娟3, 黄琴伟1, 徐靖4, 张文婷1
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1.浙江省食品药品检验研究院, 杭州 310052;2.浙江省儿童医院, 杭州 310052;3.浙江中医药大学, 杭州 310053;4.浙江省珍稀植物药工程技术研究中心, 浙江 武义 321200
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| 摘要: |
| 目的 建立灵芝孢子油HPLC指纹图谱并鉴别其油类混淆品。方法 采用HPLC，色谱柱为ACCHROMUnitary-C18（250 mm×4.6 mm，5 μm），流动相为乙腈-异丙醇（51：49），流速为1.0 mL·min-1，柱温为30℃，以蒸发光散射检测。结果 建立了灵芝孢子油脂肪酸酯类成分HPLC指纹图谱，确定了8个共有峰，分别为三亚油酸甘油酯、1，2-二亚油酸-3-油酸甘油酯、1，2-二亚油酸-3-棕榈酸甘油酯、1，2-二油酸-3-亚油酸甘油酯、1-棕榈酸-2-油酸-3-亚油酸甘油酯、三油酸甘油酯、1，2-二油酸-3-棕榈酸甘油酯、1，2-二油酸-3-硬脂酸甘油酯。灵芝孢子油混淆品的图谱与灵芝孢子油指纹图谱有明显区别。结论 建立的灵芝孢子油脂肪酸酯类成分指纹图谱能很好地区别灵芝孢子油和其混淆品。 |
| 关键词: 灵芝孢子油 灵芝孢子油混淆品 三油酸甘油酯 指纹图谱 高效液相色谱法 |
| DOI：10.13748/j.cnki.issn1007-7693.2017.07.017 |
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| 基金项目:浙江省食品药品监管系统科技计划（2014003） |
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| Studies on Fingerprint of Oil of Ganoderma Lucidum Spore and Identification for It's Confusion |
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ZHAO Weiliang1, CHENG Xiaqian2, YAN Aijuan3, HUANG Qinwei1, XU Jing4, ZHANG Wenting1
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1.Zhejiang Institute for Food and Drug Control, Hangzhou 310052, China;2.The Children's Hospital, Zhejiang University School of Medicine, Hangzhou 310052, China;3.Zhejiang Chinese Medical University, Hangzhou 310053, China;4.Zhejiang Province Rare Plant Medicine Engineering Technology Research Center, Wuyi 321200, China
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| Abstract: |
| OBJECTIVE To establish a specific HPLC fingerprint of the oil of Ganoderma lucidum spore and apply for identifying for its confusion.METHODS The HPLC chromatography was performed on an ACCHROM Unitary-C18 column (250 mm×4.6 mm,5 μm) with the mobile phase of acetonitrile-isopropanol (51︰49).The flow rate was 1.0 mL·min-1 at 30℃ with the detection of ELSD.RESULTS The chromatographic fingerprint of aliphatic esters of oil of Ganoderma lucidum spore was established.The fingerprint contained 8 recognizable peaks of trilinolein,1,2-linolein-3-olein,1,2-linolein-3-palmitin,1,2-olein-3-linolein,1-palmitin-2-olein-3-linolein,triolein,1,2-olein-3-palmitin and 1,2-olein-3-stearin.The fingerprint of oil of Ganoderma lucidum spore was different from its confution obviously.CONCLUSION The established fingerprint can distinguish oil of Ganoderma lucidum spore from its confusion. |
| Key words: oil of Ganoderma lucidum spore confusion of oil of Ganoderma lucidum spore trilinolein fingerprint HPLC |
