基于UPLC多成分含量测定的山楂炭中试炮制工艺研究

张洪坤; 郭长达; 高贯彪; 黄玉瑶; 高验杰<sup>*</sup>; 蒿亭凤

引用本文:	张洪坤,郭长达,高贯彪,黄玉瑶,高验杰,蒿亭凤.基于UPLC多成分含量测定的山楂炭中试炮制工艺研究[J].中国现代应用药学,2017,34(10):1413-1417.
	ZHANG Hongkun,GUO Changda,GAO Guanbiao,HUANG Yuyao,GAO Yanjie,HAO Tingfeng.Processing Technology Study of Crataegi Fructus Charcoal Based on Pilot-scale Level and Multi-component Quantification by UPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2017,34(10):1413-1417.

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基于UPLC多成分含量测定的山楂炭中试炮制工艺研究
张洪坤^1,2, 郭长达¹, 高贯彪¹, 黄玉瑶², 高验杰¹, 蒿亭凤¹
1.亳州市沪谯药业有限公司, 安徽亳州 236800;2.广州市香雪制药股份有限公司, 广州 510663

摘要:

目的确定山楂炭的最佳中试炮制工艺。方法采用UPLC测定山楂及其炮制品中3种酚酸类成分（绿原酸、金丝桃苷、表儿茶素）和2种三萜酸（齐墩果酸、熊果酸）的含量。在中试水平上，以山楂炭的外观性状、有机酸和上述5种成分的含量为评价指标，对影响山楂炭炮制的因素进行考察。结果山楂炭的最佳炮制工艺参数：炒制温度为200℃，炒筒转动频率为40 Hz，炒制时间为33 min。结论所建立的评价指标能有效评价山楂炭的炮制工艺，所得工艺稳定可靠。

关键词: 山楂炭炮制工艺超高效液相色谱法

DOI：10.13748/j.cnki.issn1007-7693.2017.10.011

分类号:

基金项目:

Processing Technology Study of Crataegi Fructus Charcoal Based on Pilot-scale Level and Multi-component Quantification by UPLC

ZHANG Hongkun^1,2, GUO Changda¹, GAO Guanbiao¹, HUANG Yuyao², GAO Yanjie¹, HAO Tingfeng¹

1.Bozhou Huqiao Pharmaceutical Co., Ltd., Bozhou 236800, China;2.Guangzhou Xiangxue Pharmaceutical Co., Ltd., Guangzhou 510663, China

Abstract:

OBJECTIVE To determine the processing technology of Crataegi Fructus charcoal in the pilot-scale study. METHODS Two UPLC methods for determination of effective components in Crataegi Fructus and its processed products were developed, one for determination of three phenolic compounds (chlorogenic acid, hyperoside and epicatechin), and the other for determination of two triterpene acids (oleanolic acid and ursolic acid). Taking the appearance characters, the total content of organic acid and the content of each of the five compounds above as the indexes, the effects of several influential factors the processing technology of Crataegi Fructus charcoal was investigated in the pilot-scale. RESULTS The best processing technology of Crataegi Fructus charcoal was as follows:processing temperature 200℃, turning frequency 40 Hz, processing time 33 min. CONCLUSION Evaluation indexes can effectively evaluate processing technology of Crataegi Fructus. The processing is stable and reliable, and can be used for the actual production.

Key words: Crataegi Fructus charcoal processing technology UPLC